Search results for "Ogataea polymorpha"
showing 3 items of 3 documents
Engineering of sugar transporters for improvement of xylose utilization during high-temperature alcoholic fermentation in Ogataea polymorpha yeast
2020
Abstract Background Xylose transport is one of the bottlenecks in the conversion of lignocellulosic biomass to ethanol. Xylose consumption by the wild-type strains of xylose-utilizing yeasts occurs once glucose is depleted resulting in a long fermentation process and overall slow and incomplete conversion of sugars liberated from lignocellulosic hydrolysates. Therefore, the engineering of endogenous transporters for the facilitation of glucose-xylose co-consumption is an important prerequisite for efficient ethanol production from lignocellulosic hydrolysates. Results In this study, several engineering approaches formerly used for the low-affinity glucose transporters in Saccharomyces cerev…
Evaluation of the enhanced resistance of Ogataea (Hansenula) polymorpha to benzalkonium chloride as a resource for bioremediation technologies
2019
Abstract Benzalkonium chloride (BAC) is highly abundant in wastewaters due to its wide application in household and industrial products. The aim of this research was to compare the resistance of two genetically related strains, Ogataea polymorpha (wild type) and O. polymorpha cat8Δ (recombinant strain), to enhanced concentrations of BAC (≥ 100 mg/L) in terms of its appearance in wastewaters. Inhibition of the respiration activity of wt and cat8Δ by 100 mg/L and 300 mg/L BAC in a xylose-containing broth (4 g/L xylose) was dependent on the carbon source used for obtaining the yeast biomass through ethanol fermentation. The respiration activity of wt in a liquid broth was inhibited by BAC, whi…
Pētījumi par rauga Ogataea polymorpha rezistenci pret benzalkonija hlorīdu atkarībā no kultūras fizioloģiskā stāvokļa
2018
Benzalkonija hlorīds (BAC) ir sastopams dažādos ikdienā lietojamos produktos. Šis savienojums regulāri nonāk tieši notekūdeņos. Zināms, ka BAC ir toksisks dažādiem mikroorganismiem. Tomēr ir mikroorganismi, kuriem ir novērota rezistence un spēja degradēt BAC. Starp potenciālajiem BAC degradētājiem ir arī raugi. Ogataea polymorpha jau ir pierādījusi spējas degradēt tādas toksiskas vielas, kā formaldehīds, kā arī spēju sadarboties ar bioloģiskajām notekūdeņu attīrīšanas iekārtas dūņām. Darba mērķis bija salīdzināt O. polymorpha NCYC495 ar O. polymorpha cat8Δ izturību pret BAC paaugstinātās koncentrācijas ≥100 mg/L. Lai šo mērķi sasniegtu, tika izmantotas dažādas metodes. Barotnes sastāva iete…